Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1978-12-27
pubmed:abstractText
Sodium dodecyl sulfate/polyacrylamide gels of coated vesicles from porcine brain (mean 76% coated vesicles) show three major proteins (180,000, 125,000, and 55,000 daltons) that account for 73% of the total protein. Preparations consisting predominantly of coats (65%) have less of the 55,000-dalton protein. Clathrin (180,000 daltons) comprises 40% of the protein of a coated vesicle. Conditions of 2 M urea, 0.25 M MgCl2, or pH 7.5 disrupt the coat and solubilize clathrin. Solubilized clathrin reforms coat structures after dilution of urea or MgCl2. High-pH-solubilized clathrin reassembles after dialysis against buffer at pH 6.5 containing dithiothreitol (5 mM). Reassembled coats are predominantly clathrin.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-1063406, http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-1177317, http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-1264188, http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-13764136, http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-144139, http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-181751, http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-4182372, http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-4348786, http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-4883856, http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-5094840, http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-565792, http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-933539, http://linkedlifedata.com/resource/pubmed/commentcorrection/30086-940164
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4394-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Coated vesicles: characterization, selective dissociation, and reassembly.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.