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| pubmed-article:3006041 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:3006041 | lifeskim:mentions | umls-concept:C0598312 | lld:lifeskim |
| pubmed-article:3006041 | lifeskim:mentions | umls-concept:C0205224 | lld:lifeskim |
| pubmed-article:3006041 | lifeskim:mentions | umls-concept:C0138391 | lld:lifeskim |
| pubmed-article:3006041 | lifeskim:mentions | umls-concept:C2331150 | lld:lifeskim |
| pubmed-article:3006041 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:3006041 | pubmed:dateCreated | 1986-4-10 | lld:pubmed |
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| pubmed-article:3006041 | pubmed:abstractText | The Escherichia coli gene encoding one of the primosomal proteins, protein i, was cloned by the use of synthetic oligonucleotide probes. Nucleotide sequence analysis revealed a coding region for protein i of 537 base pairs preceded by a possible promoter sequence. The gene is located adjacent to the dnaC locus, probably both being in a single operon. The protein i gene was shown to be closely related to the dnaT locus based on the following observations. (i) A multicopy plasmid carrying only the protein i gene suppresses the temperature-sensitive phenotype of a dnaT strain and restores the ability of the strain to carry out stable DNA replication in the absence of protein synthesis. (ii) An extract from a dnaT strain does not support replication of the plasmid pBR322 in vitro; addition of purified protein i restores its activity. These results indicate that protein i is encoded by dnaT and that it is essential for chromosomal DNA replication and is involved in the induction of stable DNA replication during the SOS response. | lld:pubmed |
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| pubmed-article:3006041 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:3006041 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3006041 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3006041 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:3006041 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:3006041 | pubmed:author | pubmed-author:AraiKK | lld:pubmed |
| pubmed-article:3006041 | pubmed:author | pubmed-author:MasaiHH | lld:pubmed |
| pubmed-article:3006041 | pubmed:author | pubmed-author:BondM WMW | lld:pubmed |
| pubmed-article:3006041 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3006041 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:3006041 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3006041 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3006041 | pubmed:pagination | 1256-60 | lld:pubmed |
| pubmed-article:3006041 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:3006041 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3006041 | pubmed:articleTitle | Cloning of the Escherichia coli gene for primosomal protein i: the relationship to dnaT, essential for chromosomal DNA replication. | lld:pubmed |
| pubmed-article:3006041 | pubmed:publicationType | Journal Article | lld:pubmed |
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