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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1986-4-10
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pubmed:databankReference |
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pubmed:abstractText |
The Escherichia coli gene encoding one of the primosomal proteins, protein i, was cloned by the use of synthetic oligonucleotide probes. Nucleotide sequence analysis revealed a coding region for protein i of 537 base pairs preceded by a possible promoter sequence. The gene is located adjacent to the dnaC locus, probably both being in a single operon. The protein i gene was shown to be closely related to the dnaT locus based on the following observations. (i) A multicopy plasmid carrying only the protein i gene suppresses the temperature-sensitive phenotype of a dnaT strain and restores the ability of the strain to carry out stable DNA replication in the absence of protein synthesis. (ii) An extract from a dnaT strain does not support replication of the plasmid pBR322 in vitro; addition of purified protein i restores its activity. These results indicate that protein i is encoded by dnaT and that it is essential for chromosomal DNA replication and is involved in the induction of stable DNA replication during the SOS response.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-1095769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-2991240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-363684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-4598299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6109282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6212928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6224135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6250947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6262328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6262762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6262798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6278471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6292203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6316346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6323420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6444725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6447874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6453123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-6454139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3006041-765476
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1256-60
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:3006041-Bacterial Proteins,
pubmed-meshheading:3006041-Cloning, Molecular,
pubmed-meshheading:3006041-DNA Replication,
pubmed-meshheading:3006041-Escherichia coli,
pubmed-meshheading:3006041-Gene Expression Regulation,
pubmed-meshheading:3006041-Genes,
pubmed-meshheading:3006041-Genes, Bacterial,
pubmed-meshheading:3006041-Genetic Linkage,
pubmed-meshheading:3006041-Plasmids
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pubmed:year |
1986
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pubmed:articleTitle |
Cloning of the Escherichia coli gene for primosomal protein i: the relationship to dnaT, essential for chromosomal DNA replication.
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pubmed:publicationType |
Journal Article
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