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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1986-4-11
|
| pubmed:abstractText |
Burkitt's lymphoma (BL) biopsy cells and derived cell lines can be grouped according to their patterns of reactivity with 6 selected monoclonal antibodies (MAbs) against B cell-associated surface antigens. Group I cells react only with MAbs J5 and 38.13, recognising the common acute lymphoblastic leukaemia antigen and a BL-associated antigen respectively; group II cells react with J5 and 38.13 and with one or more of a set of MAbs (Ki-24, MHM6, AC2, Ki-1) against "lymphoblastoid" antigens; group III cells react only with these anti-"lymphoblastoid" MAbs. Tumour biopsy cells from 17 cases of sporadic BL, 9 positive for the Epstein-Barr (EB) virus genome and 8 negative, have been analysed during the process of cell line establishment in vitro. In early passage the EB virus-negative BL cells showed either a group I phenotype or gave an additional reactivity with MAb Ki-24 which placed them in group II; these phenotypes remained essentially stable with continued growth of the cell lines for up to 50 passages. By contrast the EB virus-positive BL cells were much more susceptible to phenotypic change in vitro. Although such cells displayed a group I or group II phenotype in early passage, many of the lines soon moved into group III whilst retaining the karyotypic markers indicative of their malignant origin. These observations suggest that a resident EB virus genome can drive the in vitro progression of BL cells towards a more "lymphoblastoid" phenotype. This was confirmed in subsequent experiments where virus-negative BL cell lines were converted to EB virus positivity by in vitro infection. Clearly, therefore, phenotypic analysis of long-established lines can lead to false distinctions being drawn between the EB virus-positive and -negative forms of sporadic BL; both may derive from the same sub-population of target B cells in vivo.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Epstein-Barr Virus Nuclear Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Complement 3d,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Virus
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0020-7136
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
37
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
367-73
|
| pubmed:dateRevised |
2007-7-24
|
| pubmed:meshHeading |
pubmed-meshheading:3005176-Adolescent,
pubmed-meshheading:3005176-Adult,
pubmed-meshheading:3005176-Antibodies, Monoclonal,
pubmed-meshheading:3005176-Antigens, Surface,
pubmed-meshheading:3005176-Antigens, Viral,
pubmed-meshheading:3005176-Burkitt Lymphoma,
pubmed-meshheading:3005176-Cell Line,
pubmed-meshheading:3005176-Child,
pubmed-meshheading:3005176-Child, Preschool,
pubmed-meshheading:3005176-Epstein-Barr Virus Nuclear Antigens,
pubmed-meshheading:3005176-Female,
pubmed-meshheading:3005176-Genes, Viral,
pubmed-meshheading:3005176-Herpesvirus 4, Human,
pubmed-meshheading:3005176-Humans,
pubmed-meshheading:3005176-Male,
pubmed-meshheading:3005176-Middle Aged,
pubmed-meshheading:3005176-Phenotype,
pubmed-meshheading:3005176-Receptors, Complement 3d,
pubmed-meshheading:3005176-Receptors, Virus
|
| pubmed:year |
1986
|
| pubmed:articleTitle |
Epstein-Barr virus status and tumour cell phenotype in sporadic Burkitt's lymphoma.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|