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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1986-3-14
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pubmed:abstractText |
The first example of a calmodulin-like activity in a Gram-positive bacterium, Bacillus subtilis, is reported. A calcium ion-dependent, 3', 5' cyclic-AMP phosphodiesterase-stimulating activity was found in the soluble fraction of cell-free extracts of cells sporulating in a chemically-defined medium; activation was reversed by trifluoperazine. The activity was heat stable, bound to phenothiazine-agarose in a calcium ion-dependent manner and was eluted therefrom with buffer containing EGTA, and displaced authentic beef brain calmodulin from its antibody in a radioimmunoassay.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
134
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
212-7
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:3004429-3',5'-Cyclic-AMP Phosphodiesterases,
pubmed-meshheading:3004429-Animals,
pubmed-meshheading:3004429-Bacillus subtilis,
pubmed-meshheading:3004429-Calcium,
pubmed-meshheading:3004429-Calmodulin,
pubmed-meshheading:3004429-Cattle,
pubmed-meshheading:3004429-Myocardium,
pubmed-meshheading:3004429-Radioimmunoassay,
pubmed-meshheading:3004429-Trifluoperazine
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pubmed:year |
1986
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pubmed:articleTitle |
Calmodulin-like protein from Bacillus subtilis.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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