Linked Life Data

3002310

Source:http://linkedlifedata.com/resource/pubmed/id/3002310

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1986-1-30
pubmed:abstractText
Elicited, mouse peritoneal exudate cells were fractionated by centrifugation on discontinuous Percoll density gradients. Two subpopulations of neutrophils, each of greater than 90% purity, were isolated at discontinuous density gradient interfaces different from the region of mononuclear cell enrichment (i.e., 1.0694-1.0871 and 1.0872-1 X 1002 g/ml for neutrophils and less than 1.0694 g/ml for mononuclear cells). Peritoneal exudate cells were mixed with Proteus mirabilis in the presence of 1% normal mouse serum for 30 min. The mixtures were fractionated on gradients of Percoll diluted with a calcium-free medium. Populations of cells banding at densities greater than 1.0693 g/ml were washed free of gradient material, and neutrophil suspensions containing intracellular bacteria and which were relatively free of extracellular bacteria were isolated. Less than 7% of the total bacteria present was extracellular. The continuing extracellular presence of a heat-labile component of normal mouse serum was essential for maximal intracellular kill of P. mirabilis by mouse peritoneal neutrophils.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0004-945X
pubmed:author
pubmed:issnType
Print
pubmed:volume
63 ( Pt 4)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
361-70
pubmed:dateRevised
2008-6-25
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Evaluation of intracellular killing of bacteria by enriched populations of mouse peritoneal exudate neutrophils.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't