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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
1986-2-7
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pubmed:databankReference |
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pubmed:abstractText |
Human apolipoprotein B-100 (apo B-100) is the major apolipoprotein of low density lipoproteins and the principal ligand for interaction with the low density lipoprotein receptor. The human apo B-100 gene has been inserted into a lambda gt-11 expression vector, and the apo B-100 cDNA clones have been identified by screening with a monospecific apo B-100 antiserum, by screening with synthetic oligonucleotides based on the amino acid sequence of peptides isolated from apo B-100, and by immunoblot analysis of the expressed protein with a monoclonal antibody to apo B-100. The complete nucleotide and derived-amino acid sequence of a 1.7-kilobase cDNA clone of apo B-100 was determined. The 560-amino acid residues of apo B-100 contain no unique linear or repeating sequences of amino acids. The computer-predicted conformation of the apo B-100 protein contains segments of helical structure; however, a large portion of the protein is organized into beta-structure. The beta-structure may be important in lipid-apo B-100 interactions in low density lipoprotein and may contribute to the insolubility of delipidated apo B-100 in aqueous buffers. RNA blot hybridization analysis of liver mRNA utilizing a Nco I/HindIII apo B-100 cDNA probe revealed that the apo B-100 mRNA is 15-18 kilobases long, which is of sufficient size to code for a 250-387 kDa apolipoprotein, the proposed molecular size of delipidated plasma apo B-100. The gene for human apo B-100 has been localized to chromosome 2 by filter hybridization of human-mouse somatic cell hybrids utilizing a 400-base-pair Nco I/HindIII apo B-100 cDNA probe. This location is in contrast to the low density lipoprotein receptor that has been localized to chromosome 19. The cloning of human apo B-100 has provided new insights into the structure and physicochemical properties of apo B-100 and will facilitate studies on the factors modulating apo B-100 biosynthesis and the expression of the apo B-100 gene in patients with dyslipoproteinemias.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-164619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-180553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-197883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-201162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-216702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-265521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-3986194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-4107917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-4362643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6183259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6183385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6198645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6219389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6275391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6303423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6326146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6342527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6373369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6422932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6437698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6564130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6823569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-6846977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-7068636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-7169215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3001697-911786
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8340-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3001697-Amino Acid Sequence,
pubmed-meshheading:3001697-Apolipoproteins B,
pubmed-meshheading:3001697-Base Sequence,
pubmed-meshheading:3001697-Chromosome Mapping,
pubmed-meshheading:3001697-Chromosomes, Human, 1-3,
pubmed-meshheading:3001697-Cloning, Molecular,
pubmed-meshheading:3001697-DNA,
pubmed-meshheading:3001697-DNA Restriction Enzymes,
pubmed-meshheading:3001697-Humans,
pubmed-meshheading:3001697-Liver,
pubmed-meshheading:3001697-Nucleic Acid Hybridization,
pubmed-meshheading:3001697-RNA, Messenger
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pubmed:year |
1985
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pubmed:articleTitle |
Human apolipoprotein B-100: cloning, analysis of liver mRNA, and assignment of the gene to chromosome 2.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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