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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1986-1-6
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pubmed:abstractText |
The S region of the murine major histocompatibility complex contains two structurally related genes (21-OHase A and 21-OHase B) that encode 21-hydroxylase (21-OHase), an enzyme essential for the synthesis of adrenal steroids. Expression of these two genes has been analyzed by using oligonucleotide probes specific for the 21-OHase A and B genes and by DNA-mediated gene transfer. Hybridization of the oligonucleotides to blots of BALB/c adrenal RNA demonstrated that all 21-OHase mRNA is derived from the 21-OHase A gene. Cosmids bearing either the 21-OHase A or B gene were introduced into Y1 adrenocortical tumor cells by cotransfection with pSV2-neo. Cells transfected with the 21-OHase A gene expressed 21-OHase as determined by steroid metabolism and by RNA blot hybridization; 21-OHase transcripts were not detected in parent Y1 cells or in cells transfected with the 21-OHase B gene. Treatment of 21-OHase A transfectants with adrenocorticotropin increased 21-OHase mRNA levels by up to 10-fold, thus mimicking the observed effect of this hormone on 21-OHase levels in primary adrenal cultures. The regulated expression of the 21-OHase A gene in transfected Y1 cells should provide a useful system for the investigation of factors controlling the adrenal-specific regulation of 21-OHase activity.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-185050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-194704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-221910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-2983330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-4207731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-4292485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-4301573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-4311571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-4389087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-5930699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-6095106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-6286831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-6295879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-6298215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-6306344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-6308008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-6313163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-6316336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-6319383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-6334310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-6767716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-6830757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-6857266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2999780-987581
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7860-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2999780-Adrenal Cortex Neoplasms,
pubmed-meshheading:2999780-Adrenal Glands,
pubmed-meshheading:2999780-Adrenocorticotropic Hormone,
pubmed-meshheading:2999780-Animals,
pubmed-meshheading:2999780-Cloning, Molecular,
pubmed-meshheading:2999780-Gene Expression Regulation,
pubmed-meshheading:2999780-Macromolecular Substances,
pubmed-meshheading:2999780-Mice,
pubmed-meshheading:2999780-RNA, Messenger,
pubmed-meshheading:2999780-Steroid 21-Hydroxylase,
pubmed-meshheading:2999780-Steroid Hydroxylases,
pubmed-meshheading:2999780-Transcription, Genetic,
pubmed-meshheading:2999780-Transfection
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pubmed:year |
1985
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pubmed:articleTitle |
Expression of murine 21-hydroxylase in mouse adrenal glands and in transfected Y1 adrenocortical tumor cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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