rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1985-12-13
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pubmed:abstractText |
Two established human tumor cell lines, epidermoid carcinoma line A431 and glioblastoma line SF268, were studied to compare the interaction of each with epidermal growth factor (EGF). SF268 cells bound [125I] EGF with 35-40 fold higher affinity than did the A431 cells. The EGF binding sites of both lines were photoaffinity labeled using 2,4-NAPS-[125I] EGF, a photoreactive derivative of EGF. Extracts of photolysed cells analyzed by SDS-PAGE showed a difference between the two cell lines in the high molecular weight component corresponding to the EGF receptor. EGF in a dose range from 0.3-200 nM had no effect on thymidine incorporation by SF268 cells, whereas thymidine incorporation by A431 cells was markedly inhibited by EGF.
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pubmed:grant |
|
pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Oct
|
pubmed:issn |
0006-291X
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
132
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
284-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2998356-Affinity Labels,
pubmed-meshheading:2998356-Binding Sites,
pubmed-meshheading:2998356-Carcinoma, Squamous Cell,
pubmed-meshheading:2998356-Cell Line,
pubmed-meshheading:2998356-Chromatography, Gel,
pubmed-meshheading:2998356-Chromatography, High Pressure Liquid,
pubmed-meshheading:2998356-DNA Replication,
pubmed-meshheading:2998356-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2998356-Epidermal Growth Factor,
pubmed-meshheading:2998356-Glioma,
pubmed-meshheading:2998356-Humans,
pubmed-meshheading:2998356-Photochemistry,
pubmed-meshheading:2998356-Receptor, Epidermal Growth Factor,
pubmed-meshheading:2998356-Receptors, Cell Surface
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pubmed:year |
1985
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pubmed:articleTitle |
Epidermal growth factor receptors in the human glioblastoma cell line SF268 differ from those in epidermoid carcinoma cell line A431.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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