Linked Life Data

2998069

Source:http://linkedlifedata.com/resource/pubmed/id/2998069

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1985-12-2
pubmed:abstractText
To determine whether viral functions are necessary for recombination of the pseudorabies virus genome in infected cells, we have used as a model system marker rescue at the permissive temperature (PT) and nonpermissive temperature (NPT) of a temperature sensitive mutant (tsG1) deficient in the immediate-early (180K) protein. Two restriction fragments, both of which can rescue tsG1 at the PT but only one of which encompasses the whole immediate-early gene and can complement tsG1, were compared for their ability to rescue the mutant at the NPT. Although both restriction fragments rescued the mutant with equal frequency at the PT, only the fragment which could express the immediate-early 180K protein prior to recombination, i.e. could complement tsG1, rescued the mutant at the NPT. We conclude that the expression of viral functions is necessary for high frequency recombination of the pseudorabies virus genome.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
147
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-40
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
The expression of viral functions is necessary for recombination of a herpesvirus (pseudorabies).
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.