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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1985-12-13
|
| pubmed:abstractText |
ATPase and calcium binding activities were studied in sarcolemmal membranes from hearts of male rats fed either a control or 2% cholesterol diet for different time periods. Studies with isolated membrane revealed a significant increase in Na+-K+ ATPase activity, sialic acid content and ATP-independent calcium binding capacity in the presence of 1.25 mM CaCl2 in the 6 week cholesterol fed group. By 12 weeks, Na+-K+ ATPase, Mg2+-ATPase and Ca2+-ATPase activities as well as ATP-independent calcium binding in the presence of 0.05 mM CaCl2 were increased in membranes from cholesterol fed rats. A significant increase (P less than 0.05) in the sarcolemmal cholesterol/phospholipid molar ratio, which is an indicator of a decrease in membrane fluidity, was also noted in the 12 week cholesterol fed group. Concanavalin A, which is believed to decrease membrane fluidity, stimulated both Mg2+ and Ca2+-dependent ATPase activities and increased ATP-independent calcium binding in control sarcolemmal preparations and these changes resembled those observed in the sarcolemma from cholesterol fed rats. Since concanavalin A did not alter the activity of Na+-K+ ATPase, it appears that some of the observed differences in sarcolemmal activities upon cholesterol feeding did not correlate well with changes in membrane order. At 24 weeks, there was a generalized depression in the sarcolemmal ATPase activities of the cholesterol group; both Mg2+ ATPase and Ca2+ ATPase were significantly less than in control.(ABSTRACT TRUNCATED AT 250 WORDS)
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Ca(2 ) Mg(2 )-ATPase,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Transporting ATPases,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol, Dietary,
http://linkedlifedata.com/resource/pubmed/chemical/N-Acetylneuraminic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Sialic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Exchanging ATPase
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| pubmed:status |
MEDLINE
|
| pubmed:issn |
0828-282X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
1
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
194-200
|
| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:2996727-Adenosine Triphosphatases,
pubmed-meshheading:2996727-Animals,
pubmed-meshheading:2996727-Ca(2+) Mg(2+)-ATPase,
pubmed-meshheading:2996727-Calcium,
pubmed-meshheading:2996727-Calcium-Transporting ATPases,
pubmed-meshheading:2996727-Cholesterol,
pubmed-meshheading:2996727-Cholesterol, Dietary,
pubmed-meshheading:2996727-Coronary Disease,
pubmed-meshheading:2996727-Enzyme Activation,
pubmed-meshheading:2996727-Male,
pubmed-meshheading:2996727-Membrane Fluidity,
pubmed-meshheading:2996727-Myocardium,
pubmed-meshheading:2996727-N-Acetylneuraminic Acid,
pubmed-meshheading:2996727-Phospholipids,
pubmed-meshheading:2996727-Rats,
pubmed-meshheading:2996727-Rats, Inbred Strains,
pubmed-meshheading:2996727-Sarcolemma,
pubmed-meshheading:2996727-Sialic Acids,
pubmed-meshheading:2996727-Sodium-Potassium-Exchanging ATPase
|
| pubmed:articleTitle |
Heart sarcolemmal ATPase and calcium binding activities in rats fed a high cholesterol diet.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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