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rdf:type |
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lifeskim:mentions |
umls-concept:C0005186,
umls-concept:C0016030,
umls-concept:C0017337,
umls-concept:C0020792,
umls-concept:C0026882,
umls-concept:C0080194,
umls-concept:C0086418,
umls-concept:C0205409,
umls-concept:C1373200,
umls-concept:C1546465,
umls-concept:C1705175,
umls-concept:C1705176,
umls-concept:C1705177,
umls-concept:C1705178,
umls-concept:C1705294,
umls-concept:C1882348
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pubmed:issue |
20
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pubmed:dateCreated |
1985-11-18
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pubmed:abstractText |
We have sequenced the mutant beta-actin gene of a tumorigenic human fibroblast cell line (HuT-14T) and found that it carries three mutations that alter the amino acids at positions 36, 83, and 244 as well as a 22-base-pair "insertion" sequence, in the 5' intron, not present in a wild-type gene. The less tumorigenic cell line HuT-14, a progenitor of HuT-14T, has the same codon-244 mutation and the insertion sequence but not the other two mutations. A nontumorigenic cell line that is related to HuT-14 but that has no beta-actin mutations does carry the intron-length polymorphism. We conclude that the mutation at codon 244 occurred first in a beta-actin allele already bearing the 22-base-pair intron insert and that mutations at codons 36 and 83 arose subsequently during the selection for the HuT-14T phenotype. Rat-2 cells synthesize the appropriate charge-variant species of mutant actin protein when transfected with either the singly or the triply mutated beta-actin gene.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-165499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-265521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-418410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-4277645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6095033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6147349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6201399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6269744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6286831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6315714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6322116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6332316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6352074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6386181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6499833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6646124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6722823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6892569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-6893954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-7067038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-7199389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2996000-7269249
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6995-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2996000-Actins,
pubmed-meshheading:2996000-Base Sequence,
pubmed-meshheading:2996000-Cell Line,
pubmed-meshheading:2996000-Cell Transformation, Neoplastic,
pubmed-meshheading:2996000-Cloning, Molecular,
pubmed-meshheading:2996000-Codon,
pubmed-meshheading:2996000-DNA Transposable Elements,
pubmed-meshheading:2996000-Fibroblasts,
pubmed-meshheading:2996000-Genes,
pubmed-meshheading:2996000-Humans,
pubmed-meshheading:2996000-Lymphoma,
pubmed-meshheading:2996000-Mutation
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pubmed:year |
1985
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pubmed:articleTitle |
Identification and order of sequential mutations in beta-actin genes isolated from increasingly tumorigenic human fibroblast strains.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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