| pubmed-article:2995973 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2995973 | lifeskim:mentions | umls-concept:C0035366 | lld:lifeskim |
| pubmed-article:2995973 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:2995973 | lifeskim:mentions | umls-concept:C0019425 | lld:lifeskim |
| pubmed-article:2995973 | lifeskim:mentions | umls-concept:C0079868 | lld:lifeskim |
| pubmed-article:2995973 | lifeskim:mentions | umls-concept:C0002085 | lld:lifeskim |
| pubmed-article:2995973 | lifeskim:mentions | umls-concept:C1521840 | lld:lifeskim |
| pubmed-article:2995973 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:2995973 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:2995973 | pubmed:dateCreated | 1985-10-31 | lld:pubmed |
| pubmed-article:2995973 | pubmed:abstractText | A clonal murine cell line that is heterozygous at the beta 2-microglobulin locus (B2m) was obtained by Abelson murine leukemia virus (Ab-MuLV) transformation of liver cells from (C57BL/6 X BALB/c) F1 fetuses. To obtain proviral insertional mutants, we superinfected a subclone of these cells, which does not express the env surface protein of the Moloney leukemia virus (Mo-MuLV, the helper virus that was used to transmit the defective Ab-MuLV genome during transformation), with Mo-MuLV. Mutant clones that fail to express the C57BL/6 allele of B2m (B2mb) were then immunoselected by using a monoclonal antibody that specifically recognizes the B2mb gene product and not that of the B2ma allele. Of 22 independent clones obtained, one contains a proviral insertion that is near or in the first exon of the B2mb gene. Surprisingly, the insertion is of the Ab-MuLV genome and not of replication-competent Mo-MuLV. This indicates that superinfection with Mo-MuLV "rescued" the defective Ab-MuLV genome, which then inserted into the B2mb gene. We conclude that when an allele-specific selection procedure exists, proviral insertion is a potential method for obtaining mutations in heterozygous mammalian cells. This approach may thereby provide a method for molecular cloning of such selectable genes, using a retroviral hybridization probe. | lld:pubmed |
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| pubmed-article:2995973 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2995973 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2995973 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2995973 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2995973 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:2995973 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:2995973 | pubmed:author | pubmed-author:LeonWW | lld:pubmed |
| pubmed-article:2995973 | pubmed:author | pubmed-author:RosenbergNN | lld:pubmed |
| pubmed-article:2995973 | pubmed:author | pubmed-author:PotterT ATA | lld:pubmed |
| pubmed-article:2995973 | pubmed:author | pubmed-author:RajanT VTV | lld:pubmed |
| pubmed-article:2995973 | pubmed:author | pubmed-author:FrankelWW | lld:pubmed |
| pubmed-article:2995973 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2995973 | pubmed:volume | 82 | lld:pubmed |
| pubmed-article:2995973 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2995973 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2995973 | pubmed:pagination | 6600-4 | lld:pubmed |
| pubmed-article:2995973 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2995973 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2995973 | pubmed:articleTitle | Retroviral insertional mutagenesis of a target allele in a heterozygous murine cell line. | lld:pubmed |
| pubmed-article:2995973 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2995973 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2995973 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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