Linked Life Data

2995430

Source:http://linkedlifedata.com/resource/pubmed/id/2995430

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1985-11-12
pubmed:abstractText
The ability of endocrine organs to express human immune response-associated antigens (Ia), such as HLA-DR, is a subject of intense current interest. In this study, the effects of various potential modulators of thyroid follicular cell HLA-DR expression were examined using in vitro cultures. A culture supernatant containing T-cell-derived lymphokines caused DR antigen expression on 13-18% of thyroid cells; more consistent effects were produced by recombinant gamma-interferon, which led to 46-100% of the thyroid cells becoming HLA-DR positive after 3 days in culture. This effect was both time and concentration dependent and occurred in thyroid cells derived from patients with Graves' disease (n = 7) and Hashimoto's thyroiditis (n = 2) as well as from three subjects with no autoimmune thyroid disease. Thyroid cells stained with the monoclonal antibodies 4F2 and 5E9, which recognize cell activation antigens, regardless of whether they were treated with gamma-interferon. The lectin phytohemagglutinin also induced HLA-DR antigen expression (21-91% of cells positive). This response was dependent on T cell contamination of thyroid cell suspensions, since the effect was inhibited by cyclosporin A. HLA-DQ antigen expression, identified by the Leu-10 monoclonal antibody, was also induced on thyroid cells by gamma-interferon and phytohemagglutinin. In contrast, neither recombinant alpha-interferon nor interleukin-2 induced HLA-DR antigens. Irradiation reduced the response of thyroid cells to gamma-interferon, but two of the known inhibitors of macrophage Ia expression, prostaglandin E2 and (Bu)2cAMP, did not affect gamma-interferon-induced thyroid cell HLA-DR expression. We were unable to detect interleukin-1 production by thyroid cells. These results suggest that 1) under normal circumstances, thyroid cells are 4F2 and 5E9 positive, but are incapable of expressing Ia antigens and, thus, of activating T cells to initiate autoimmune thyroiditis; and 2) once activated, for example by a virus, T cells could release gamma-interferon and induce thyroid cell HLA-DR and -DQ antigen expression; these Ia-positive thyroid cells could then have a role in maintaining or enhancing the autoimmune response.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-972X
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
817-24
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:2995430-Adult, pubmed-meshheading:2995430-Aged, pubmed-meshheading:2995430-Bucladesine, pubmed-meshheading:2995430-Cells, Cultured, pubmed-meshheading:2995430-Cyclosporins, pubmed-meshheading:2995430-Dinoprostone, pubmed-meshheading:2995430-Female, pubmed-meshheading:2995430-Graves Disease, pubmed-meshheading:2995430-HLA-DQ Antigens, pubmed-meshheading:2995430-HLA-DR Antigens, pubmed-meshheading:2995430-Histocompatibility Antigens Class II, pubmed-meshheading:2995430-Humans, pubmed-meshheading:2995430-Interferon-gamma, pubmed-meshheading:2995430-Interleukin-1, pubmed-meshheading:2995430-Lymphokines, pubmed-meshheading:2995430-Male, pubmed-meshheading:2995430-Middle Aged, pubmed-meshheading:2995430-Prostaglandins E, pubmed-meshheading:2995430-T-Lymphocytes, pubmed-meshheading:2995430-Thyroid Gland, pubmed-meshheading:2995430-Thyroiditis, Autoimmune
pubmed:year
1985
pubmed:articleTitle
The in vitro regulation of human thyrocyte HLA-DR antigen expression.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't