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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1985-11-13
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pubmed:abstractText |
Human thyroid peroxidase (TPO) has been purified from thyroid microsomes by immunoaffinity chromatography using a monoclonal antibody (mAb) to TPO. The eluted material had a specific activity of 381 U/mg and exhibited a peak in the Soret region. The ratio of A411 to A280 ranged from 0.20 to 0.25. Upon SDS-polyacrylamide gel electrophoresis, the purified enzyme gave two contiguous bands in the 100 kDa region. Further, it has been demonstrated that sera with anti-microsomal autoantibodies from patients presenting Graves' or Hashimoto's thyroiditis diseases were able to bind to purified TPO and to inhibit in a dose-dependent manner the mAb binding to purified TPO. This suggests that TPO is the thyroid antigen termed to date the microsomal antigen.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
190
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
147-52
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2995127-Antibodies, Monoclonal,
pubmed-meshheading:2995127-Antigens,
pubmed-meshheading:2995127-Autoantigens,
pubmed-meshheading:2995127-Autoimmune Diseases,
pubmed-meshheading:2995127-Chemical Phenomena,
pubmed-meshheading:2995127-Chemical Precipitation,
pubmed-meshheading:2995127-Chemistry,
pubmed-meshheading:2995127-Chromatography, Affinity,
pubmed-meshheading:2995127-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2995127-Humans,
pubmed-meshheading:2995127-Immunochemistry,
pubmed-meshheading:2995127-Isoenzymes,
pubmed-meshheading:2995127-Microsomes,
pubmed-meshheading:2995127-Peroxidase,
pubmed-meshheading:2995127-Peroxidases,
pubmed-meshheading:2995127-Solubility,
pubmed-meshheading:2995127-Thyroid Diseases,
pubmed-meshheading:2995127-Thyroid Gland
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pubmed:year |
1985
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pubmed:articleTitle |
Purification of the human thyroid peroxidase and its identification as the microsomal antigen involved in autoimmune thyroid diseases.
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pubmed:publicationType |
Journal Article
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