Linked Life Data

2993634

Source:http://linkedlifedata.com/resource/pubmed/id/2993634

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1985-9-27
pubmed:abstractText
When the herbicide paraquat (1,1'-dimethyl-4,4'-bipyridylium) was administered to adult rat pulmonary alveolar macrophages (PAM) in primary culture, both a time-dependent and a dose-dependent cytotoxic response (cell death) was observed. An LD50 value of 1 mM was calculated when these cells were exposed to paraquat in vitro for 12 h in Ham's F12 culture medium at 30 degrees C. Cell death was accompanied by the formation of TBA-reactive substances (lipid peroxidation) and was potentiated by hyperoxia (95% O2). In a 95% O2-5% CO2 atmosphere, an LD50 value of 0.1 mM was calculated. In addition, the presence of superoxide dismutase in the culture medium (1700 units/ml) inhibited the cytotoxic response. Since [14C]paraquat was not absorbed into these cells, extracellular superoxide anion radical formation was investigated as the cause of the observed cell death. Paraquat (0.5 mM) was found to stimulate extracellular O-2 generation, from PAM, but only in nonactivated cells. A sevenfold enhancement over the resting rate of radical generation was observed in the presence of paraquat. No increase in the O-2 generation rate of activated macrophages was observed upon the addition of paraquat to the culture medium. These data indicate that paraquat is cytotoxic to the pulmonary alveolar macrophage and further suggest that this cytotoxicity is mediated, at least in part, by an excess, extracellular production of active oxygen species. Implications of these findings with respect to the currently accepted hypothesis of paraquat poisoning in vivo are discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0098-4108
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
417-29
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Paraquat toxicity in vitro. I. Pulmonary alveolar macrophages.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.