| pubmed-article:2991748 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2991748 | lifeskim:mentions | umls-concept:C0004038 | lld:lifeskim |
| pubmed-article:2991748 | lifeskim:mentions | umls-concept:C0439851 | lld:lifeskim |
| pubmed-article:2991748 | lifeskim:mentions | umls-concept:C0439852 | lld:lifeskim |
| pubmed-article:2991748 | lifeskim:mentions | umls-concept:C0872178 | lld:lifeskim |
| pubmed-article:2991748 | lifeskim:mentions | umls-concept:C1552596 | lld:lifeskim |
| pubmed-article:2991748 | lifeskim:mentions | umls-concept:C1947931 | lld:lifeskim |
| pubmed-article:2991748 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:2991748 | pubmed:dateCreated | 1985-9-4 | lld:pubmed |
| pubmed-article:2991748 | pubmed:abstractText | We performed three sets of experiments to determine whether cloned DNA fragments can be substituted for homologous regions of the Aspergillus nidulans genome by DNA-mediated transformation. A linear DNA fragment containing a heteromorphic trpC+ allele was used to transform a trpC- strain to trpC+. Blot analysis of DNA from the transformants showed that the heteromorphic allele had replaced the trpC- allele in a minority of the strains. An A. nidulans trpC+ gene was inserted into the argB+ gene, and a linear DNA fragment containing the resultant null argB allele was used to transform a trpC- argB+ strain to trpC+. Approximately 30% of the transformants were simultaneously argB-. The null argB allele had replaced the wild-type allele in a majority of these strains. The A. nidulans SpoC1 C1-C gene was modified by removal of an internal restriction fragment and introduced into a trpC- strain by transformation with a circular plasmid. A transformant containing a tandem duplication of the C1-C region separated by plasmid DNA was self-fertilized, and trpC- progeny were selected. All of these had lost the introduced plasmid DNA sequences, whereas about half had retained the modified C1-C gene and lost the wild-type copy. Thus, it is possible with A. nidulans to replace chromosomal DNA sequences with DNA fragments that have been cloned and modified in vitro by using either one- or two-step procedures similar to those developed for Saccharomyces cerevisiae. | lld:pubmed |
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| pubmed-article:2991748 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:2991748 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2991748 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2991748 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:2991748 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:2991748 | pubmed:author | pubmed-author:MillerB LBL | lld:pubmed |
| pubmed-article:2991748 | pubmed:author | pubmed-author:TimberlakeW... | lld:pubmed |
| pubmed-article:2991748 | pubmed:author | pubmed-author:MillerK YKY | lld:pubmed |
| pubmed-article:2991748 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2991748 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:2991748 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2991748 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2991748 | pubmed:pagination | 1714-21 | lld:pubmed |
| pubmed-article:2991748 | pubmed:dateRevised | 2010-9-9 | lld:pubmed |
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| pubmed-article:2991748 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2991748 | pubmed:articleTitle | Direct and indirect gene replacements in Aspergillus nidulans. | lld:pubmed |
| pubmed-article:2991748 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2991748 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2991748 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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