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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1985-9-4
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pubmed:abstractText |
We performed three sets of experiments to determine whether cloned DNA fragments can be substituted for homologous regions of the Aspergillus nidulans genome by DNA-mediated transformation. A linear DNA fragment containing a heteromorphic trpC+ allele was used to transform a trpC- strain to trpC+. Blot analysis of DNA from the transformants showed that the heteromorphic allele had replaced the trpC- allele in a minority of the strains. An A. nidulans trpC+ gene was inserted into the argB+ gene, and a linear DNA fragment containing the resultant null argB allele was used to transform a trpC- argB+ strain to trpC+. Approximately 30% of the transformants were simultaneously argB-. The null argB allele had replaced the wild-type allele in a majority of these strains. The A. nidulans SpoC1 C1-C gene was modified by removal of an internal restriction fragment and introduced into a trpC- strain by transformation with a circular plasmid. A transformant containing a tandem duplication of the C1-C region separated by plasmid DNA was self-fertilized, and trpC- progeny were selected. All of these had lost the introduced plasmid DNA sequences, whereas about half had retained the modified C1-C gene and lost the wild-type copy. Thus, it is possible with A. nidulans to replace chromosomal DNA sequences with DNA fragments that have been cloned and modified in vitro by using either one- or two-step procedures similar to those developed for Saccharomyces cerevisiae.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-13040135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-159454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-16593541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-291033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-347451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-388424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-4896022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6220717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6230518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6251546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6273866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6281139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6289435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6296830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6302667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6307979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6308623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6310324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6312834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6318083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6324178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6324193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6328272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6353203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6353245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6363209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6363619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6368319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6392570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6449291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-6997115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-7034956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991748-922889
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1714-21
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:2991748-Aspergillus nidulans,
pubmed-meshheading:2991748-Cell Differentiation,
pubmed-meshheading:2991748-Chromosome Mapping,
pubmed-meshheading:2991748-DNA, Fungal,
pubmed-meshheading:2991748-DNA Restriction Enzymes,
pubmed-meshheading:2991748-Gene Expression Regulation,
pubmed-meshheading:2991748-Genes, Fungal,
pubmed-meshheading:2991748-Plasmids,
pubmed-meshheading:2991748-Transformation, Genetic
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pubmed:year |
1985
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pubmed:articleTitle |
Direct and indirect gene replacements in Aspergillus nidulans.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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