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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1985-9-4
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pubmed:databankReference |
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pubmed:abstractText |
The his4-917 mutation of Saccharomyces cerevisiae results from the insertion of the Ty element Ty917 into the regulatory region of the HIS4 gene and renders the cell His-. The hist4-912 delta mutant, which carries a solo delta in the 5'-noncoding region of HIS4, is His+ at 37 degrees C but His- at 23 degrees C. Both these mutations interfere with HIS4 expression at the transcriptional level. The His- phenotype of both insertion mutations is suppressed by mutations at the SPT2 locus. The product of the wild-type SPT2 gene apparently represses HIS4 transcription in these mutant strains; this repression is relieved when the SPT2 gene is destroyed by mutation. The repression of transcription by SPT2 presumably results from an interaction between the SPT2+ gene product and Ty or delta sequences. In this paper, we report the cloning and DNA sequence analysis of the wild-type SPT2 gene and show that the gene is capable of encoding a protein of 333 amino acids in length. In addition, we show that a dominant mutation of the SPT2 gene results from the generation of an ochre codon which is presumed to lead to a shortened SPT2 gene product.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-222467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-347451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-378400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-395030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6095055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6145588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6154870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6212926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6235151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6250062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6250712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6250713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6251544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6260957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6261152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6266279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6266752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6268990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6273866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6291054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6295879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6300868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6305507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6308768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6310323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6321953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6326126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6329902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6330560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6330566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6345791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-642007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6429549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6452580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6571216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6616616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6760802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6886377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6896364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-6997135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-7034964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-7037777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-7063407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991744-7088190
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1543-53
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2991744-Amino Acid Sequence,
pubmed-meshheading:2991744-Chromosome Mapping,
pubmed-meshheading:2991744-Cloning, Molecular,
pubmed-meshheading:2991744-Codon,
pubmed-meshheading:2991744-DNA, Fungal,
pubmed-meshheading:2991744-DNA Restriction Enzymes,
pubmed-meshheading:2991744-DNA Transposable Elements,
pubmed-meshheading:2991744-Fungal Proteins,
pubmed-meshheading:2991744-Gene Expression Regulation,
pubmed-meshheading:2991744-Genes, Fungal,
pubmed-meshheading:2991744-Genes, Regulator,
pubmed-meshheading:2991744-Histidine,
pubmed-meshheading:2991744-Mutation,
pubmed-meshheading:2991744-Transcription, Genetic
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pubmed:year |
1985
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pubmed:articleTitle |
Isolation and characterization of the SPT2 gene, a negative regulator of Ty-controlled yeast gene expression.
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pubmed:publicationType |
Journal Article
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