|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1985-8-27
|
|
pubmed:abstractText |
Polyclonal and monoclonal antibodies to individual herpes simplex virus (HSV) glycoproteins were tested for ability to inhibit adsorption of radiolabeled HSV type 1 (HSV-1) strain HFEMsyn [HSV-1(HFEM)syn] to HEp-2 cell monolayers. Polyclonal rabbit antibodies specific for glycoprotein D (gD) or gC and three monoclonal mouse antibodies specific for gD-1 or gC-1 most effectively inhibited HSV-1 adsorption. Antibodies of other specificities had less or no inhibitory activity despite demonstrable binding of the antibodies to virions. Nonimmune rabbit immunoglobulin G and Fc fragments partially inhibited adsorption when used at relatively high concentrations. These results suggest involvement of gD, gC, and perhaps gE (the Fc-binding glycoprotein) in adsorption. The monoclonal anti-gD antibodies that were most effective at inhibiting HSV-1 adsorption had only weak neutralizing activity. The most potent anti-gD neutralizing antibodies had little effect on adsorption at concentrations significantly higher than those required for neutralization. This suggests that, although some anti-gD antibodies can neutralize virus by blocking adsorption, a more important mechanism of neutralization by anti-gD antibodies may be interference with a step subsequent to adsorption, possibly penetration.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-14100619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-14200087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-14434282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-171455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-176453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-186433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-221669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-227992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-229267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-2991571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-4110104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-4289440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-4297604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-4351780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-4362401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-4369085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6090699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6092678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6093367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6097034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6187935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6194310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6199891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6246278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6249749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6260657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6270896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6281979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6283107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6283108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6284965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6292453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6310880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6321760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6323754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6328323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6345804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6358356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-6402547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-7142712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2991570-80458
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fab Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fc Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/glycoprotein D, Human herpesvirus 1,
http://linkedlifedata.com/resource/pubmed/chemical/glycoprotein gC, herpes simplex...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
55
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
475-82
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2991570-Adsorption,
pubmed-meshheading:2991570-Antibodies, Monoclonal,
pubmed-meshheading:2991570-Antibodies, Viral,
pubmed-meshheading:2991570-Antibody Specificity,
pubmed-meshheading:2991570-Cell Membrane,
pubmed-meshheading:2991570-Humans,
pubmed-meshheading:2991570-Immunoglobulin Fab Fragments,
pubmed-meshheading:2991570-Immunoglobulin Fc Fragments,
pubmed-meshheading:2991570-Immunoglobulin G,
pubmed-meshheading:2991570-Neutralization Tests,
pubmed-meshheading:2991570-Simplexvirus,
pubmed-meshheading:2991570-Viral Envelope Proteins,
pubmed-meshheading:2991570-Viral Proteins
|
|
pubmed:year |
1985
|
|
pubmed:articleTitle |
Specificities of monoclonal and polyclonal antibodies that inhibit adsorption of herpes simplex virus to cells and lack of inhibition by potent neutralizing antibodies.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
