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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1985-9-16
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pubmed:abstractText |
Direct biochemical evidence for the existence of mutations in five of the RNA segments of the A/Leningrad/134/57 cold-adapted 47th passage mutant as compared with its wild-type progenitor has been obtained using two techniques. T1 oligonucleotide mapping of total viral RNA as well as of individual RNA segments revealed changes in RNAs 4, 5 and 6. Analysis of S1 nuclease-treated RNA-RNA hybrids on polyacrylamide gels revealed changes in at least one of the polymerase genes as well as in RNAs 4, 5, 6 and 7. These findings provide a direct demonstration for the existence of multiple mutations in the cold-adapted mutant vaccine strain.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
66 ( Pt 8)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1697-704
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2991435-Cold Temperature,
pubmed-meshheading:2991435-Endonucleases,
pubmed-meshheading:2991435-Influenza A virus,
pubmed-meshheading:2991435-Mutation,
pubmed-meshheading:2991435-Nucleic Acid Hybridization,
pubmed-meshheading:2991435-Oligoribonucleotides,
pubmed-meshheading:2991435-RNA, Viral,
pubmed-meshheading:2991435-Ribonuclease T1,
pubmed-meshheading:2991435-Single-Strand Specific DNA and RNA Endonucleases,
pubmed-meshheading:2991435-Species Specificity
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pubmed:year |
1985
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pubmed:articleTitle |
Comparative studies of A/Leningrad/134/57 wild-type and 47-times passaged cold-adapted mutant influenza viruses: oligonucleotide mapping and RNA-RNA hybridization studies.
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pubmed:publicationType |
Journal Article,
Comparative Study
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