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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1985-9-4
|
| pubmed:abstractText |
We have mapped a transcriptional regulatory sequence within the 1.0 kb noncoding region of the bovine papilloma virus (BPV-1) genome, using an enhancer dependent expression vector for chloramphenicol acetyltransferase. This transcriptional regulatory element works independently of position and orientation, and its function is significantly augmented in BPV-1 transformed C127 cells and in monkey CV-1 cells acutely transfected with plasmids expressing BPV-1 early gene products. Using defined deletion mutants of the BPV-1 DNA and full-length viral cDNAs expressed from an SV40 early promoter, we demonstrate that the expression of this trans-activating factor maps to the 3' open reading frames of the viral transforming region. A premature termination codon engineered into the E2 ORF eliminates expression of this diffusible transactivation function establishing the E2 gene product as the diffusible trans-activating factor.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0092-8674
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
42
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
183-91
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:2990724-Acetyltransferases,
pubmed-meshheading:2990724-Animals,
pubmed-meshheading:2990724-Bovine papillomavirus 1,
pubmed-meshheading:2990724-Cattle,
pubmed-meshheading:2990724-Cell Line,
pubmed-meshheading:2990724-Cell Transformation, Neoplastic,
pubmed-meshheading:2990724-Cell Transformation, Viral,
pubmed-meshheading:2990724-Cercopithecus aethiops,
pubmed-meshheading:2990724-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:2990724-DNA, Recombinant,
pubmed-meshheading:2990724-DNA Replication,
pubmed-meshheading:2990724-Enhancer Elements, Genetic,
pubmed-meshheading:2990724-Genes, Regulator,
pubmed-meshheading:2990724-Genes, Viral,
pubmed-meshheading:2990724-Mice,
pubmed-meshheading:2990724-Papillomaviridae,
pubmed-meshheading:2990724-Plasmids,
pubmed-meshheading:2990724-Promoter Regions, Genetic,
pubmed-meshheading:2990724-Transcription, Genetic,
pubmed-meshheading:2990724-Transfection,
pubmed-meshheading:2990724-Virus Replication
|
| pubmed:year |
1985
|
| pubmed:articleTitle |
Transactivation of a bovine papilloma virus transcriptional regulatory element by the E2 gene product.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|