Linked Life Data

2988892

Source:http://linkedlifedata.com/resource/pubmed/id/2988892

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-8-12
pubmed:abstractText
Incubation of a HeLa cell fraction termed P1000 with genomic DNA fragments containing portions of the 5'-flanking region of the ovalbumin gene, followed by digestion with exonucleases, shows that this cell fraction contains a protein (or proteins) that binds to the distal promoter of the ovalbumin gene. The protein protects both strands and spans a minimum of 22 bp on the noncoding strand from nucleotides -68 to -90. The 5' border of the protected region on the coding strand is located at nucleotide -81. A similar site on the chick beta-globin gene is also protected by HeLa cell fraction P1000. The exonuclease footprinting methods used to identify the distal promoter-binding protein are more sensitive than the DNaseI footprinting method, and can be used to identify sequence-specific binding proteins in a mixture of DNA binding proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0198-0238
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
233-40
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Identification by exonuclease footprinting of a distal promoter-binding protein from HeLa cell extracts.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.