Linked Life Data

2988256

Source:http://linkedlifedata.com/resource/pubmed/id/2988256

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1985-7-16
pubmed:abstractText
A recently described method to investigate growth hormone (GH) receptors on circulating human blood cells has been used to study the effect of a trypsin preparation and antitrypsin moieties on hormone binding to lymphocytes. Trypsinization with one defined dose of trypsin (10 ng/ml) led to a considerable decrease both of specific binding and of binding affinity (affinity constant after 60 min trypsinization 0.5 X 10(9) vs 1.5 X 10(9) M-1 in untreated control cells). Exposure of peripheral blood lymphocytes (PBL) to antitrypsin activities was followed by a steady increase of affinity and specific binding (affinity constant: with 10 KIU anti-trypsin 1.9 X 10(9) M-1, with 100 KIU 2.4 X 10(9) M-1, with 1000 KIU 3.6 X 10(9) M-1). This antitrypsin effect exceeds the binding values expected after blocking trypsin activities in the incubation medium. In a subset of experiments somatomedin-B (SM-B) was used as the antitrypsin moiety and was shown to increase specific GH binding to PBL in a similar manner as did antitrypsin (with 1000 ng SM-B affinity constant (Ka) 12.0 X 10(9) M-1, specific binding 9.7% of total radioactivity). It is concluded that enzymatic factors and their inhibitors including partially GH dependent moieties like SM-B modulate specific GH binding to human peripheral lymphocytes in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0001-5598
pubmed:author
pubmed:issnType
Print
pubmed:volume
109
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
139-44
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Effect of enzyme and enzyme inhibitors on specific binding of hGH to human peripheral lymphocytes.
pubmed:publicationType
Journal Article