Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1985-7-22
pubmed:abstractText
Large tumor (T) antigen and its bound multimeric states are positioned by scanning transmission electron microscopy (STEM) within a few base pairs at control sequences of the simian virus 40 DNA origin of replication region. Proximal and distal edge positions for each multimer group match the end positions of previously mapped fragments protected from DNase cleavage. Since chance correspondence is shown to be extremely unlikely, STEM mass measurements, obtained concurrently with STEM map positions, indicate that the DNase fragments arise from bound monomers, dimers, trimers, and tetramers in binding region II and monomers, dimers, and trimers in binding region I. Simultaneous binding of seven monomer-equivalent masses is observed, three in region I and four in region II, with an ordered and interpretable mass distribution in the plane of the foil. Although this observation does not prove that the six G-A-G-G-C and one T-A-G-G-C sequences, similarly distributed, function as recognition sequences for T-antigen monomer, it provides strong support for such a model. The stable existence in solution of low-and intermediate-mass structures, observed at lower T-antigen concentrations, suggests a role as assembly intermediates.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-163375, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-166203, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-184459, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-226310, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-230499, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6101224, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6248243, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6256739, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6259538, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6269743, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6269805, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6270901, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6270902, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6273580, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6283167, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6288959, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6288963, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6292510, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6293767, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6296253, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6298451, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6298452, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6301686, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6310127, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6313230, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6319005, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6329720, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6461415, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6757448, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-6955791, http://linkedlifedata.com/resource/pubmed/commentcorrection/2987937-7338923
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3626-30
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Monomers through trimers of large tumor antigen bind in region I and monomers through tetramers bind in region II of simian virus 40 origin of replication DNA as stable structures in solution.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.