Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1985-6-20
|
| pubmed:databankReference | |
| pubmed:abstractText |
We have cloned the human alpha 1-antitrypsin (alpha 1-AT) gene and identified the promoter and the transcription initiation point. The cloned gene, following transfection, is expressed in a cell-specific manner, being transcribed in a human hepatoma cell line (Hep3B) but not in HeLa cells. We show that the 5' flanking region of the alpha 1-AT gene contains DNA sequences sufficient for efficient transcription in Hep3B but not in HeLa cells. This DNA sequence also activates, in a cell-specific manner, heterologous promoters such as that of SV40; however, the effect is only obtained in one orientation, suggesting that this cis-acting cell-specific element does not share all the features generally associated with enhancers. By cotransfection-competition experiments we also show the existence of a limiting trans-acting factor, essential for the expression of the alpha 1-AT gene in Hep3B cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0092-8674
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
41
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
531-40
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:2985281-Base Sequence,
pubmed-meshheading:2985281-Carcinoma, Hepatocellular,
pubmed-meshheading:2985281-Cell Line,
pubmed-meshheading:2985281-Cloning, Molecular,
pubmed-meshheading:2985281-DNA,
pubmed-meshheading:2985281-Enhancer Elements, Genetic,
pubmed-meshheading:2985281-Gene Expression Regulation,
pubmed-meshheading:2985281-HeLa Cells,
pubmed-meshheading:2985281-Humans,
pubmed-meshheading:2985281-Liver,
pubmed-meshheading:2985281-Liver Neoplasms,
pubmed-meshheading:2985281-Promoter Regions, Genetic,
pubmed-meshheading:2985281-RNA, Messenger,
pubmed-meshheading:2985281-RNA Splicing,
pubmed-meshheading:2985281-Transcription, Genetic,
pubmed-meshheading:2985281-Transcription Factors,
pubmed-meshheading:2985281-Transfection,
pubmed-meshheading:2985281-alpha 1-Antitrypsin
|
| pubmed:year |
1985
|
| pubmed:articleTitle |
Cell-specific expression of a transfected human alpha 1-antitrypsin gene.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|