Linked Life Data

2984534

Source:http://linkedlifedata.com/resource/pubmed/id/2984534

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-5-2
pubmed:abstractText
A new method for preparation of viable islet cells from the normal pancreas is introduced. After total pancreatectomy, the pancreatic duct is cannulated and perfused with Hanks' balanced salt solution (HBSS) containing 0.2% collagenase at 37 degrees C for 30-45 minutes. The gland is then chopped and dissociated by shaking in a water bath. The cell suspension is filtered through steel mesh and washed with cold HBSS by centrifugation. By this method, collagenase is applied preferentially into the acinar tissue and the islet yield is greatly enhanced with decreased acinar contamination. Estimation of insulin and amylase in pancreatic tissue and islet rich cell suspension (graft) indicates a 57% islet recovery and a six-fold enrichment in islet concentration. Graft prepared by this method is autotransplanted into totally pancreatectomised dogs and normoglycemia is achieved in ten of 13 animals.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0301-4894
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
209-15
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
[A new method for preparation of islet cells from dogs].
pubmed:publicationType
Journal Article, English Abstract