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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1992-4-22
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pubmed:abstractText |
Malaria sporozoite infection rates in a mixed species group of 244 Anopheles gambiae Giles sensu lato and 115 An.funestus Giles wild female mosquitoes were compared using three methods to determine cut-off absorbance values for positivity of a Plasmodium falciparum Welch enzyme-linked immunosorbent assay (ELISA). Positive controls were based on P.falciparum circumsporozoite protein. As negative controls, four wild male Anopheles were included on each microtitre plate; tests were repeated on four consecutive days for each plate. Infection rates were estimated at 13.1-22.8% using the mean absorbance value of negative controls plus three standard deviations, 11.7-12.8% using double the mean and 12.5-13.6% using the fixed cut-off value of 0.20 (allowing for 20% variation in negative control absorbance values). Observed agreement for positivity or negativity among samples tested four times was 98.6% for the 2 x mean method, 97.2% for the fixed cut-off 0.20 value, but only 82.7% for the mean + 3 SD method. It was concluded that the 2x mean cut-off method is most reliable for field studies. P.falciparum sporozoite rates of 12.2% in An.funestus and 11.9% in An.gambiae s.l. were thus determined on the basis of the 2x mean cut-off method. This comparative evaluation demonstrates that vector infectivity rates can be seriously over-estimated from sporozoite ELISA tests, by as much as 87% in one case considered here, depending on the absorbance cut-off method applied for negative controls.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0269-283X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
259-64
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2980182-Animals,
pubmed-meshheading:2980182-Anopheles,
pubmed-meshheading:2980182-Antigens, Protozoan,
pubmed-meshheading:2980182-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2980182-Female,
pubmed-meshheading:2980182-Insect Vectors,
pubmed-meshheading:2980182-Male,
pubmed-meshheading:2980182-Plasmodium falciparum,
pubmed-meshheading:2980182-Predictive Value of Tests,
pubmed-meshheading:2980182-Protozoan Proteins
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pubmed:year |
1988
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pubmed:articleTitle |
ELISA absorbance cut-off method affects malaria sporozoite rate determination in wild Afrotropical Anopheles.
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pubmed:affiliation |
Biomedical Sciences Research Centre, Kenya Medical Research Institute, Nairobi.
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pubmed:publicationType |
Journal Article,
Comparative Study
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