Linked Life Data

2973844

Source:http://linkedlifedata.com/resource/pubmed/id/2973844

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-1-18
pubmed:abstractText
The heat-stable antigen (HSA), recognized by the monoclonal antibodies M1/69, B2A2, and J11d, is low or absent on the surface of most murine peripheral T cells but present on all but 3% of thymocytes. The CD4-CD8+ and CD4+CD8- or "single positive" thymic populations may be divided into further subgroups based on surface HSA expression. One group, CD4-CD8+ and expressing very high levels of HSA (HSA++), is an immature, T cell antigen receptor (TcR) negative, outer cortical blast cell. However, a further subdivision of CD4-CD8+ and CD4+CD8- single positives may be made, into those negative to low for HSA (HSA-) and those expressing moderate amounts of HSA (HSA+). The proportion of HSA- single positives is low in the thymus of young mice, whereas the proportion of HSA+ single positives is similar to that of the adult. Both the HSA- and the HSA+ subsets of single positive thymocytes from adult mice are CD3+ and express the normal peripheral T cell incidence of V beta 8 determinants on the TcR. On stimulation with concanavalin A in limit-dilution culture both HSA- and HSA+ subsets of single positive thymocytes give a high frequency of proliferating clones, and the clones from both HSA- and HSA+ subsets of CD4-CD8+ thymocytes are cytotoxic. Thus both HSA- and HSA+ single positive thymocytes are functionally mature. The HSA- subsets of single positive thymocytes differ from the HSA+ subsets in being slightly larger in size, in expressing higher levels of MEL-14, in binding more peanut agglutinin, and in including a proportion of cells expressing high levels of the Pgp-1 glycoprotein. It is suggested that HSA- CD4-CD8+ and HSA- CD4+CD8- thymocytes are more mature than their HSA+ counterparts, and might represent a previously activated or "memory" thymic subpopulation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0008-8749
pubmed:author
pubmed:issnType
Print
pubmed:volume
117
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
312-26
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:2973844-Animals, pubmed-meshheading:2973844-Antibodies, Monoclonal, pubmed-meshheading:2973844-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:2973844-Antigens, Surface, pubmed-meshheading:2973844-Cell Differentiation, pubmed-meshheading:2973844-Cell Survival, pubmed-meshheading:2973844-Complement System Proteins, pubmed-meshheading:2973844-Flow Cytometry, pubmed-meshheading:2973844-Fluorescent Antibody Technique, pubmed-meshheading:2973844-Hot Temperature, pubmed-meshheading:2973844-Male, pubmed-meshheading:2973844-Mice, pubmed-meshheading:2973844-Mice, Inbred CBA, pubmed-meshheading:2973844-Receptors, Antigen, T-Cell, pubmed-meshheading:2973844-T-Lymphocytes, pubmed-meshheading:2973844-T-Lymphocytes, Helper-Inducer, pubmed-meshheading:2973844-T-Lymphocytes, Regulatory
pubmed:year
1988
pubmed:articleTitle
Subpopulations of mature murine thymocytes: properties of CD4-CD8+ and CD4+CD8- thymocytes lacking the heat-stable antigen.
pubmed:affiliation
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't