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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1989-1-10
|
| pubmed:abstractText |
Employing osmotically shocked lysate of a spectinomycin resistant strain of Escherichia coli, trospectomycin, a new alkylspectinomycin, was adenylylated in the presence of adenosine 5'-triphosphate and magnesium ion. A highly resistant strain of E. coli was obtained by transforming a laboratory strain with a newly constructed plasmid consisting of pBR322 and a determinant for spectinomycin resistance originally found on a low copy number plasmid in E. coli strain NR79. The biologically inactive adenylylated trospectomycin was found to be trospectomycin 6-(5'-adenylate).
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0021-8820
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
41
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1452-61
|
| pubmed:dateRevised |
2006-5-1
|
| pubmed:meshHeading |
pubmed-meshheading:2973455-Adenosine Monophosphate,
pubmed-meshheading:2973455-Cell-Free System,
pubmed-meshheading:2973455-Drug Resistance,
pubmed-meshheading:2973455-Escherichia coli,
pubmed-meshheading:2973455-Hydrolysis,
pubmed-meshheading:2973455-Magnetic Resonance Spectroscopy,
pubmed-meshheading:2973455-Microbial Sensitivity Tests,
pubmed-meshheading:2973455-Plasmids,
pubmed-meshheading:2973455-Recombination, Genetic,
pubmed-meshheading:2973455-Spectinomycin
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Adenylylation of trospectomycin by crude enzyme preparations from Escherichia coli.
|
| pubmed:affiliation |
Upjohn Company, Kalamazoo, Michigan 49001.
|
| pubmed:publicationType |
Journal Article
|