Linked Life Data

2972860

Source:http://linkedlifedata.com/resource/pubmed/id/2972860

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-12-12
pubmed:abstractText
Administration of antibody, directed against glomerular basement membrane (GBM) heparan sulfate-proteoglycan, into a presensitized rat results in the induction of membranous nephropathy with subepithelial immune-complex deposits. In this investigation, we examined the mechanisms responsible for the formation of subepithelial immune-complex deposits in the anti-HS-PG model. In initial experiments, the intravenously administered radioiodinated antibody was seen exclusively localized in the regions of the glomerular capillary wall where the subepithelial deposits were observed. To determine their exclusive localization in the subepithelial space, kinetics of movement of the intravenously administered antibody was investigated. The antibody localized in the inner layers of the GBM within a few minutes after its administration. It equilibrated in the inner and outer layers of the GBM in a matter of a few hours. Then, after 24 hours, it gradually disappeared from the inner layers of the GBM and persisted in the outer layers only. The ready clearance of the antibody from the inner layers may be related to the differential in the kinetics of lateral intrinsic plasma fluid currents within the GBM. The persistence of heterologous antibody exclusively in the outer layers and the availability of host autologous antibodies probably resulted in the development of immune complex deposits in the subepithelial space. The glomeruli devoid of plasma water currents showed no change in the concentration of the antibody in the inner and outer layers of the GBM or mesangial matrix. Also, no antibody binding was observed with the plasmalemma of either the foot processes or visceral epithelia. The data suggest that the biochemical-biophysical properties of the glomerular capillary wall, in concert with its intraglomerular hemodynamics, most likely played a significant role in the development of subepithelial immune-complex deposits in this model.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0085-2538
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
209-19
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:2972860-Animals, pubmed-meshheading:2972860-Antigen-Antibody Complex, pubmed-meshheading:2972860-Basement Membrane, pubmed-meshheading:2972860-Binding Sites, Antibody, pubmed-meshheading:2972860-Chondroitin Sulfate Proteoglycans, pubmed-meshheading:2972860-Disease Models, Animal, pubmed-meshheading:2972860-Female, pubmed-meshheading:2972860-Glomerulonephritis, Membranoproliferative, pubmed-meshheading:2972860-Glycosaminoglycans, pubmed-meshheading:2972860-Heparan Sulfate Proteoglycans, pubmed-meshheading:2972860-Heparitin Sulfate, pubmed-meshheading:2972860-Immune Complex Diseases, pubmed-meshheading:2972860-Immunization, pubmed-meshheading:2972860-Immunoglobulin G, pubmed-meshheading:2972860-Kidney Glomerulus, pubmed-meshheading:2972860-Proteoglycans, pubmed-meshheading:2972860-Rats, pubmed-meshheading:2972860-Rats, Inbred F344
pubmed:year
1988
pubmed:articleTitle
Nephritogenicity of proteoglycans. III. Mechanism of immune deposit formation.
pubmed:affiliation
Department of Pathology, Northwestern University Medical School, Chicago, Illinois.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.