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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
19
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pubmed:dateCreated |
1988-7-27
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pubmed:abstractText |
A seryl protease which catalyzes conversion of proatrial natriuretic factor (ANF) to the active circulating form, ANF(99-126), was purified from a particulate fraction of bovine atria. The enzyme was solubilized with 1.6 M KCl. The molecular mass of the purified enzyme was 580 kDa on gel filtration, whereas by sodium dodecyl sulfate-polyacrylamide gel electrophoresis a cluster of six bands with molecular masses around 30 kDa was observed. The purified enzyme produced ANF(99-126) from partially purified bovine pro-ANF by the selective cleavage of the arginyl peptide bond in the -Pro97-Arg98-Ser99-sequence in pro-ANF. The enzyme was localized mainly in the microsomal fraction rather than the granule fraction. It is likely that the enzyme selectively cleaves the Arg98-Ser99 peptide bond in pro-ANF during the process of secretion.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
263
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9515-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2967825-Animals,
pubmed-meshheading:2967825-Atrial Natriuretic Factor,
pubmed-meshheading:2967825-Cattle,
pubmed-meshheading:2967825-Heart Atria,
pubmed-meshheading:2967825-Molecular Weight,
pubmed-meshheading:2967825-Myocardium,
pubmed-meshheading:2967825-Protease Inhibitors,
pubmed-meshheading:2967825-Protein Precursors,
pubmed-meshheading:2967825-Protein Processing, Post-Translational,
pubmed-meshheading:2967825-Serine Endopeptidases,
pubmed-meshheading:2967825-Subcellular Fractions
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pubmed:year |
1988
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pubmed:articleTitle |
Atrioactivase, a specific peptidase in bovine atria for the processing of pro-atrial natriuretic factor. Purification and characterization.
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pubmed:affiliation |
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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