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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1980-8-15
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pubmed:abstractText |
GM 126 diploid fibroblasts were fused with a heat-sensitive mouse cell mutant defective in DNA synthesis, and primary hybrids were selected at permissive and nonpermissive temperatures in HAT medium. Primary hybrids, primary hybrid clones back-selected in 8-azaguanine at the permissive temperature, and subclones of heat-resistant primary hybrids isolated under nonselective conditions or after 8-azaguanine treatment were tested for heat sensitivity, the expression of 26 human enzymes assigned to 19 different human chromosomes, and the presence of human chromosomes. Only the human X chromosome and X-linked marker enzymes exhibited a clear pattern of concordant segregation with the heat-resistant phenotype. On the basis of these observations, we have defined the human genetic locus that corrects the heat-sensitive lesion in tsC1AGOH as hrC1AGOH and have assigned this locus to the X chromosome. This observation provides the first instance where two selectable markers (heat resistance and 8-azaguanine sensitivity) are found on a single human chromosome and suggests that these markers may prove to be a valuable push-pull selective system of use in determining the linear arrangement of genes on human chromosomes by somatic cell genetics.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/296762-180537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/296762-182062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/296762-182373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/296762-322459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/296762-4345850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/296762-4366846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/296762-4587225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/296762-4689040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/296762-5160834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/296762-5271170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/296762-560242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/296762-911846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/296762-949742
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
975-96
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:296762-Animals,
pubmed-meshheading:296762-Cell Line,
pubmed-meshheading:296762-Chromosome Mapping,
pubmed-meshheading:296762-DNA,
pubmed-meshheading:296762-Female,
pubmed-meshheading:296762-Genes,
pubmed-meshheading:296762-Genetic Markers,
pubmed-meshheading:296762-Hot Temperature,
pubmed-meshheading:296762-Humans,
pubmed-meshheading:296762-Hybrid Cells,
pubmed-meshheading:296762-Mice,
pubmed-meshheading:296762-Phenotype,
pubmed-meshheading:296762-Sex Chromosomes,
pubmed-meshheading:296762-X Chromosome
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pubmed:year |
1979
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pubmed:articleTitle |
X-linkage of a human genetic locus that corrects the DNA synthesis lesion in tsC1AGOH mouse cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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