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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1988-5-20
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pubmed:abstractText |
T cells in explants of human fetal small intestine in organ culture were stimulated in situ with PWM or anti-CD3 antibody to test the hypothesis that activated T cells produce enteropathy in human small intestine. T cell activation was measured by the appearance of CD25+ cells in the lamina propria of the explants and IL-2 production into the organ culture supernatant. We have previously shown that the number of T cells in human fetal gut increased between 14 and 22 wk gestation. Accordingly, after the addition of PWM to cultured explants of fetal intestine the number of CD25+ cells in the lamina propria and the amounts of IL-2 secreted into the organ culture supernatant increased with the age of the explanted tissue. The addition of PWM also produced an age-related enteropathy, most noticeably crypt epithelial cell hyperplasia and villous atrophy, with relatively minor changes in 14-17-wk-old intestine but severe tissue damage in 18-22-wk-old fetal intestine. These enteropathic effects were also produced when mucosal T cells were activated with anti-CD3 mAb. Cyclosporin A completely inhibited the PWM-induced development of CD25+ cells and related tissue damage. These experiments show that activated T cells in human small intestine produce enteropathy. The model provides a new system with which to dissect the mechanisms of T cell-mediated intestinal damage.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965735-1083353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965735-18287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965735-25166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965735-310861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965735-3491695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965735-3536221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965735-3967805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965735-4113189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965735-4621368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965735-6334364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965735-6334715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965735-6408171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965735-648817
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
167
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1341-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2965735-Antibodies, Monoclonal,
pubmed-meshheading:2965735-Antigens, CD3,
pubmed-meshheading:2965735-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2965735-Cyclosporins,
pubmed-meshheading:2965735-Enteritis,
pubmed-meshheading:2965735-Fetus,
pubmed-meshheading:2965735-Gestational Age,
pubmed-meshheading:2965735-Humans,
pubmed-meshheading:2965735-Interleukin-2,
pubmed-meshheading:2965735-Intestinal Mucosa,
pubmed-meshheading:2965735-Intestine, Small,
pubmed-meshheading:2965735-Lymphocyte Activation,
pubmed-meshheading:2965735-Organ Culture Techniques,
pubmed-meshheading:2965735-Pokeweed Mitogens,
pubmed-meshheading:2965735-T-Lymphocytes
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pubmed:year |
1988
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pubmed:articleTitle |
Evidence that activated mucosal T cells play a role in the pathogenesis of enteropathy in human small intestine.
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pubmed:affiliation |
Department of Paediatric Gastroenterology, St. Bartholomews Hospital, London, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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