Linked Life Data

2965642

Source:http://linkedlifedata.com/resource/pubmed/id/2965642

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-5-23
pubmed:abstractText
The in vitro reactivity of purified murine Ly-2+ and L3T4+ T cells towards 2,4,6-trinitrophenyl (TNP)-modified syngeneic stimulator cells was analyzed. Both T cell subpopulations autonomously proliferated and produced interleukin 2. In either the Ly-2+ or L3T4+ T cell subset the frequencies of TNP-specific interleukin 2 (IL 2)-producing T lymphocyte precursors (IL 2 TL-p) were equally high (f = 1/400-1/1000). Clonally developing IL 2 TL of either T cell subset showed an exquisite antigen (TNP) specificity as shown by the split culture approach. TNP-specific Ly-2+ IL-2 TL used class I MHC (H-2Kk) gene products as major histocompatibility complex (MHC) restriction elements, while L3T4+ IL 2 TL proved to be class II MHC (H-2I-AkI-Ek) restricted. Clonal segregation analyses revealed that the majority of clonally developing TNP-reactive Ly-2+ TL segregated into either IL 2 TL-p or cytotoxic T lymphocyte presursors, i.e. both functions appear to be mutually exclusive. Less than 10% of the responding Ly-2+ T cells seemed to be bifunctional. These findings provide compelling evidence for the L3T4+ T cell-independent, autonomous reactivity of Ly-2+ T cells in MHC-restricted antigen-specific responses and suggest T-T cell interactions within the functional heterogenous Ly-2+ T cell population.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
325-32
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Reactivity of Ly-2+ T cells against 2,4,6-trinitrophenyl (TNP)-modified syngeneic stimulator cells: specificity, frequency of interleukin 2-producing Ly-2+ helper T cells and clonal segregation from Ly-2+ cytotoxic T lymphocytes.
pubmed:affiliation
Department of Medical Microbiology and Immunology, University of Ulm.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't