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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1988-5-12
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pubmed:abstractText |
Transfection of cells with the H-2Kk gene lacking the transmembrane and cytoplasmic segments resulted in secretion of the H-2Kk protein, as determined by immunoprecipitation with monoclonal anti-H-2Kk antibodies. Transgenic (H-2b X H-2d)F1 mice were established carrying integrated copies of the modified H-2Kk gene. Expression of the soluble H-2Kk antigen in the transgenic mice was demonstrated in cell supernatants of biosynthetically labeled splenic and thymic Con A blasts as well as bone marrow-derived macrophages. Soluble H-2Kk molecules were also present in the sera of the transgenic animals. No cell-surface expression of the H-2Kk antigen could be observed. In spite of the presence of the soluble H-2Kk molecules in the transgenic mice, the animals were able to generate H-2Kk-specific cytolytic T cells as well as antibody responses when stimulated with cell-surface-bound H-2Kk antigens. These responses were indistinguishable from those of the nontransgenic littermates. Possible explanations for the observed lack of tolerance are discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-13099277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-141046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-233910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-2413454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-2420883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-2425364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-2581256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-3491158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-3561410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-4377238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-6088078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-6096831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-6180834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-61864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-6192569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-6203969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-6369679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-6577257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-6808076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-6972991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-7009746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-7096324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-7144911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2965390-82607
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2269-73
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2965390-Animals,
pubmed-meshheading:2965390-Antibodies, Monoclonal,
pubmed-meshheading:2965390-Female,
pubmed-meshheading:2965390-H-2 Antigens,
pubmed-meshheading:2965390-Immune Tolerance,
pubmed-meshheading:2965390-Isoantibodies,
pubmed-meshheading:2965390-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:2965390-Male,
pubmed-meshheading:2965390-Mice,
pubmed-meshheading:2965390-Mice, Inbred C57BL,
pubmed-meshheading:2965390-Mice, Inbred DBA,
pubmed-meshheading:2965390-Mice, Transgenic,
pubmed-meshheading:2965390-Solubility,
pubmed-meshheading:2965390-T-Lymphocytes, Cytotoxic
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pubmed:year |
1988
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pubmed:articleTitle |
Alloreactive immune responses of transgenic mice expressing a foreign transplantation antigen in a soluble form.
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pubmed:affiliation |
Institute for Immunology and Genetics, German Cancer Research Center, Heidelberg.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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