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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
1988-3-2
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pubmed:abstractText |
Laser desorption mass spectrometry has been employed for the structural determination of lipid A components derived from the lipopolysaccharides (LPS) of gram-negative bacteria. Mass spectra were obtained for methylated monophosphoryl lipid A from Neisseria gonorrhoeae and Rhodopseudomonas sphaeroides, for diphosphoryl lipid A from Escherichia coli and for the intact LPS from the Re Mutant of E. coli consisting of triphosphoryl lipid A and two KDO (2-keto-3-deoxyoctonate) units. Fragmentation of the phosphate (or pyrophosphate) on the reducing glucosamine is followed by fragmentation of acyl-linked fatty acids. Also observed are fragment ions which correspond to the distal portion of the molecule.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0887-6134
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
591-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2962661-Escherichia coli,
pubmed-meshheading:2962661-Gram-Negative Bacteria,
pubmed-meshheading:2962661-Lasers,
pubmed-meshheading:2962661-Lipid A,
pubmed-meshheading:2962661-Lipopolysaccharides,
pubmed-meshheading:2962661-Mass Spectrometry,
pubmed-meshheading:2962661-Neisseria gonorrhoeae,
pubmed-meshheading:2962661-Rhodobacter sphaeroides
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pubmed:year |
1987
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pubmed:articleTitle |
Structural determination of lipid A from gram negative bacteria using laser desorption mass spectrometry.
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pubmed:affiliation |
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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