Linked Life Data

2960679

Source:http://linkedlifedata.com/resource/pubmed/id/2960679

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
34
pubmed:dateCreated
1988-1-12
pubmed:abstractText
The cytosolic fraction of insulin-treated adipocytes exhibits a 2-fold increase in protein kinase activity when Kemptide is used as a substrate. The detection of insulin-stimulated kinase activity is critically dependent on the presence of phosphatase inhibitors such as fluoride and vanadate in the cell homogenization buffer. The cytosolic protein kinase activity exhibits high sensitivity (ED50 = 2 X 10(-10) M) and a rapid response (maximal after 2 min) to insulin. Kinetic analyses of the cytosolic kinase indicate that insulin increases the Vmax of Kemptide phosphorylation and ATP utilization without affecting the affinities of this enzyme toward the substrate or nucleotide. Upon chromatography on anion-exchange and gel filtration columns, the insulin-stimulated cytosolic kinase activity is resolved from the cAMP-dependent protein kinase and migrates as a single peak with an apparent Mr = 50,000-60,000. The partially purified kinase preferentially utilizes histones, Kemptide, multifunctional calmodulin-dependent protein kinase substrate peptide, ATP citrate-lyase, and acetyl-coenzyme A carboxylase as substrates but does not catalyze phosphorylation of ribosomal protein S6, casein, phosvitin, phosphorylase b, glycogen synthase, inhibitor II, and substrate peptides for casein kinase II, protein kinase C, and cGMP-dependent protein kinase. Phosphoamino acid analyses of the 32P-labeled substrates reveal that the insulin-stimulated cytosolic kinase is primarily serine-specific. The insulin-activated cytosolic kinase prefers Mn2+ to Mg2+ and is independent of Ca2+. Unlike ribosomal protein S6 kinase and protease-activated kinase II, the insulin-sensitive cytosolic kinase is fluoride-insensitive. Taken together, these results indicate that a novel cytosolic protein kinase activity is activated by insulin.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
262
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
16677-85
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:2960679-Adipose Tissue, pubmed-meshheading:2960679-Animals, pubmed-meshheading:2960679-Chromatography, Ion Exchange, pubmed-meshheading:2960679-Cytosol, pubmed-meshheading:2960679-Dithiothreitol, pubmed-meshheading:2960679-Dose-Response Relationship, Drug, pubmed-meshheading:2960679-Heparin, pubmed-meshheading:2960679-Hydrogen-Ion Concentration, pubmed-meshheading:2960679-Insulin, pubmed-meshheading:2960679-Insulin Antibodies, pubmed-meshheading:2960679-Male, pubmed-meshheading:2960679-Manganese, pubmed-meshheading:2960679-Oligopeptides, pubmed-meshheading:2960679-Protein Kinases, pubmed-meshheading:2960679-Protein-Serine-Threonine Kinases, pubmed-meshheading:2960679-Rats, pubmed-meshheading:2960679-Rats, Inbred Strains, pubmed-meshheading:2960679-Substrate Specificity, pubmed-meshheading:2960679-Temperature
pubmed:year
1987
pubmed:articleTitle
Insulin stimulates a novel Mn2+-dependent cytosolic serine kinase in rat adipocytes.
pubmed:affiliation
Department of Biochemistry, University of Massachusetts Medical School, Worcester 01605.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't