Linked Life Data

2960606

Source:http://linkedlifedata.com/resource/pubmed/id/2960606

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1987-12-31
pubmed:abstractText
Fluorescein-conjugated monoclonal antibodies (mAb) have been used to examine the cellular distribution and genetic relationship of the RT7 and the leukocyte common antigen (L-CA) in the rat. We demonstrate here that the RT7.1 alloantigen, recognized by the mAb BC84, is present on B lymphocytes as well as T lymphocytes, although at markedly different apparent concentrations; T cells binding approximately 4 times more BC84 than do B cells. In contrast, the polymorphic L-CA recognized by the NDS58 mAb and the non-polymorphic L-CA recognized by the OX1 mAb appear to be expressed in equal concentrations on T and B cells and differ in their tissue distribution from the RT7.1 alloantigen. We have also tested a new mAb termed 8G6.1, that is reactive with RT7b rat strains and has a cell, tissue and strain distribution profile resembling that of a polymorphic L-CA. Segregation analysis of the RT7 and polymorphic L-CA antigenic systems using a single backcross model demonstrate that the alloantigens recognized by BC84 and NDS58 cosegregate and that both are allelic with respect to 8G6.1. The L-CA antigenic determinant defined by OX1 was non-polymorphic and thus was not allelic with any of the antigenic systems tested. These results suggest that there is a close genetic relationship between the expression of RT7 and L-CA in the rat.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0272-457X
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
531-43
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Monoclonal antibodies to RT7 and LCA antigens in the rat: cell distribution and segregation analysis.
pubmed:affiliation
Department of Pathology, School of Medicine, University of Connecticut Health Center, Farmington 06032.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't