Linked Life Data

2959592

Source:http://linkedlifedata.com/resource/pubmed/id/2959592

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1987-12-17
pubmed:abstractText
Expression of the bacteriophage Mu mom gene is subject to a variety of regulatory controls. Both the host Dam DNA-adenine methylase and the phage Mu C protein are required for mom gene transcription. In addition, the Mu com gene product is required for production of the mom protein. Because the com and mom genes overlap on the same mRNA transcript (with com being located proximal to the 5' end), it is likely that Com function is exerted after transcription initiation. To study the role of Com, two segments of Mu were cloned in both orientations (+ and -) into the HindIII site of the galactokinase expression vector, pKG1800; the HindIII site is located between the galK structural gene and its promoter. In (+) plasmids, the Mu DNA inserts were transcribed from the gal promoter in the same orientation as in the phage genome; (-) plasmids had the Mu DNA inserted in the reverse orientation. Each Mu insert contained the same segment of the mom gene from the 3' terminus, but differed in the extent of com gene included at the 5' terminus; one contained a truncated com gene and the other a complete com gene, as well as upstream Mu regulatory sequences. The results are summarized as follows: (1) both (-) plasmids produced only about 10% as much galactokinase activity following fucose induction as the parental vector, pKG1800; (2) plasmid pGTVH(+), with an intact com gene produced about 30% as much galactokinase as pKG1800; (3) plasmid pMTVH(+), with a truncated com gene, produced only about 10% as much enzyme as pKG1800.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
345-51
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
The bacteriophage Mu com gene appears to specify a translation factor required for mom gene expression.
pubmed:affiliation
Department of Biology, University of Rochester, NY 14627.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.