rdf:type |
|
lifeskim:mentions |
umls-concept:C0001461,
umls-concept:C0005456,
umls-concept:C0012854,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0027950,
umls-concept:C0028027,
umls-concept:C0178499,
umls-concept:C0332294,
umls-concept:C0631452,
umls-concept:C1167622,
umls-concept:C1335439,
umls-concept:C1511539,
umls-concept:C1626935
|
pubmed:issue |
8
|
pubmed:dateCreated |
1987-10-1
|
pubmed:abstractText |
The poly(adenosine diphosphoribose) polymerase activity of isolated liver nuclei was inhibited by 4-carbamoylbenzenediazonium chloride, referred to as 4-diazoniobenzamide, an effect that was dependent on the time of incubation and the concentration of the diazonium compound, with inhibition following first-order kinetics. The inhibition was not reversed by reisolation of nuclei and centrifugal washing, whereas the inhibition by benzamide or 4-aminobenzamide was completely reversible under these conditions. Simultaneous incubation of 4-diazoniobenzamide with benzamide prevented enzyme inhibition. The 4-diazoniobenzoic acid analogue was not inhibitory. The mechanism of action of 4-diazoniobenzamide was traced to a specific covalent binding to dGMP of DNA to form N2-[(4-carbamoylphenyl)azo]-2'-deoxyguanosine 5'-monophosphate. Coenzymic DNA, by tight association with the polymerase protein, fixes the -C(O)NH2 moiety of the adduct at the nicotinamide-binding site of the enzyme.
|
pubmed:grant |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0006-2960
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
21
|
pubmed:volume |
26
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2226-31
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:2956988-Animals,
pubmed-meshheading:2956988-Cattle,
pubmed-meshheading:2956988-Cell Nucleus,
pubmed-meshheading:2956988-DNA,
pubmed-meshheading:2956988-Deoxyguanosine,
pubmed-meshheading:2956988-Diazonium Compounds,
pubmed-meshheading:2956988-Indicators and Reagents,
pubmed-meshheading:2956988-Liver,
pubmed-meshheading:2956988-Niacinamide,
pubmed-meshheading:2956988-Poly(ADP-ribose) Polymerases,
pubmed-meshheading:2956988-Thymus Gland,
pubmed-meshheading:2956988-Tritium
|
pubmed:year |
1987
|
pubmed:articleTitle |
Covalent binding of 4-carbamoylbenzenediazonium chloride to deoxyguanine bases of DNA resulting in apparent irreversible inhibition of poly(adenosine diphosphoribose) polymerase at the nicotinamide binding site.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|