Linked Life Data

2956199

Source:http://linkedlifedata.com/resource/pubmed/id/2956199

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1987-9-14
pubmed:abstractText
The level of expression of the class-I major histocompatibility (MHC) antigen was determined in a series of human embryo cell lines transformed with either adenovirus 12 (Ad 12) early region I (EI) or adenovirus 5 (Ad 5) EI DNA or with combinations of Ad 12 early region IA (EIA) or Ad 2 EIA with activated N-ras DNA. MHC class-I antigen expression was greatly reduced in all Ad 12 transformants, compared to primary cells. Expression was also reduced in the Ad 5 cell lines transformed with EIA and EIB DNA, but levels were near normal in those lines with only EIA DNA present. Amounts of MHC class-I antigen on the cell surface, as determined by RIA and FACS analysis, generally reflected total cellular levels as determined by Western blotting. Expression of beta 2 microglobulin was also much reduced in those cell lines with low levels of MHC class-I antigen. The treatment of primary cells and all the transformants with human gamma-interferon resulted in increased expression of HLA on the cell surface. Infection of primary human cells with Ad 12 or with a series of Ad 12 mutants did not have any effect on the MHC class-I antigens present.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0020-7136
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
213-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
The level of expression of class-I MHC antigens in adenovirus-transformed human cell lines.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't