Linked Life Data

2955566

Source:http://linkedlifedata.com/resource/pubmed/id/2955566

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-8-5
pubmed:abstractText
In order to define functionally important regions of the E1 a gene of adenovirus 12 (Ad12), a number of Ad12 mutants were studied. These mutants share an identical 69-bp deletion in the first exon of E1a as well as duplications of a long terminal repeat sequence at the end of the Ad12 genome. The mutants are fully competent for replication and growth in their normal hosts and have a host range extended to include the Vero cell line of African green monkey origin. The partially deleted E1a can stimulate the expression of all early adenoviral genes as well as the cellular heat shock gene, the transcription of which is stimulated by Ad5 E1a. However, plasmids containing the deleted E1a plus wild type E1b were unable to effect a transformation of either primary rat embryo fibroblasts or on an established cell line following transfection. Further, the mutant viruses were defective in generating tumors in newborn hamsters. We conclude that the deleted sequence of E1a described here is critical for transformation by E1a but is not necessary for multiplication of the virus or the transcriptional activating function of the gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
159
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-56
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Definition of a region required for transformation in E1a of adenovirus 12.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't