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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1987-5-11
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pubmed:abstractText |
A general in vivo procedure for cloning Escherichia coli genes into cosmids has been developed. The method we describe here uses a deleted Mu phage (a mini-Mu) to transpose E. coli genes into cosmids during mini-Mu replication. The resulting cosmids clones are packaged in vivo into lambda phage particles. Plasmids carrying a particular DNA sequence can be selectively recovered after infection of a new host with the in vivo constructed genomic cosmid library. This system was used successfully to clone several E. coli genes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0026-8925
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
205
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
546-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2951581-Bacteriophage lambda,
pubmed-meshheading:2951581-Chromosome Deletion,
pubmed-meshheading:2951581-Cloning, Molecular,
pubmed-meshheading:2951581-Coliphages,
pubmed-meshheading:2951581-Cosmids,
pubmed-meshheading:2951581-Escherichia coli,
pubmed-meshheading:2951581-Genes, Bacterial,
pubmed-meshheading:2951581-Genotype,
pubmed-meshheading:2951581-Transduction, Genetic
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pubmed:year |
1986
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pubmed:articleTitle |
In vivo cloning of DNA into multicopy cosmids by mini-Mu-cosduction.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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