Linked Life Data

2947961

Source:http://linkedlifedata.com/resource/pubmed/id/2947961

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-1-29
pubmed:abstractText
Cultured neonatal rat myocardial cells (CMC) were incubated with 10(-8) to 10(-5) mol/L doxorubicin (ADR), dactinomycin (ACT), and plicamycin (MIT), respectively, in medium containing 35 mu Ci sulfur 35-labeled methionine to determine comparative effects on myocardial contractile protein synthesis. Cells were harvested in 2 mmol/L Tris buffer with 0.1% Triton X-100, homogenized, and fractionated by centrifugation. Aliquots of the extracts containing equivalent amounts of protein were applied to 8% to 15% gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Similar aliquots were subjected to isoelectric focusing and to gel electrophoresis in the second dimension. All electrophoretic gels were autoradiographed. A concentration-dependent effect of ADR, ACT, and MIT on protein and on actin synthesis was found in cultured myocytes with decreased density of radiolabeled actin band in CMC exposed to either 10(-5) mol/L ADR, or from 10(-8) to 10(-5) mol/L ACT, or from 10(-7) to 10(-5) mol/L MIT. Autoradiographs of two-dimensional gels focused actin isoforms but showed substantial decreases of all actin isoforms in CMC extracts from 10(-7) mol/L ACT. ACT and MIT each decreased CMC protein synthesis by 20% at 10(-8) mol/L. At 10(-6) mol/L ADR, synthesis of alpha-actin was diminished preferentially compared with beta and gamma. Synthesis of all actin isoforms was diminished at 10(-5) mol/L ADR. The effect of MIT and ACT on CMC actin and protein synthesis in heart cells is not as specific as the effect of ADR on CMC actin and protein synthesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-2143
pubmed:author
pubmed:issnType
Print
pubmed:volume
109
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
67-74
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Actin synthesis in cultured cardiac myocytes: comparative effects of doxorubicin, dactinomycin, and plicamycin.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't