Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6 Pt 2
pubmed:dateCreated
1987-2-19
pubmed:abstractText
Fibronectin was not removed from the substratum beneath focal adhesion sites when fibroblasts spread in serum-free medium on adsorbed fibronectin substrata, or when fibroblasts spread in serum-containing medium on covalently cross-linked fibronectin substrata. Under these conditions, there was colocalization between 140-kD fibronectin receptors and focal adhesion sites. It was concluded that removal of adsorbed fibronectin from beneath focal adhesion sites was a mechanical process that required serum. The effect of serum was nonspecific since serum could be replaced by equivalent concentrations of serum albumin, ovalbumin, or gamma globulins. Quantitative measurements indicated that the presence of proteins in the incubation medium weakens the interaction of fibronectin with the substratum, thereby allowing the adsorbed protein to be removed from the substratum at sites of high stress. After removing fibronectin from the substratum, cells reorganized this material into patches and fibrils beneath cells, and the reorganized fibronectin colocalized with fibronectin receptors. Some of the patches of fibronectin were phagocytosed. The fibronectin fibrils were observed to be in register with actin filament bundles and sometimes translocated to the upper cell surfaces. It is proposed that removal of fibronectin from beneath focal adhesion sites is an example of how cells can modify their extracellular matrices through contractile activity.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-208994, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-286310, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-3002832, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-3080317, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-3155652, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-365353, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-3709521, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-3884631, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-3917945, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-3921405, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-3921554, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-4450367, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-5097522, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-574428, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-575139, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-6367964, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-6423268, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-6430913, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-6540273, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-6630292, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-6767616, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-6786758, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-6791830, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-6815205, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-6933459, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-6998561, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-7002323, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-7004879, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-7023273, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-7075867, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-7076724, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-7084332, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-7174731, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-7348271, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-7419598, http://linkedlifedata.com/resource/pubmed/commentcorrection/2947902-932106
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
103
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2697-706
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Focal adhesion sites and the removal of substratum-bound fibronectin.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.