Linked Life Data

2944599

Source:http://linkedlifedata.com/resource/pubmed/id/2944599

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1986-10-30
pubmed:abstractText
Trimethyl capping of U2 snRNA has been studied using U2 genes with mutations located in either the 5' flanking or the coding region. A monomethyl (7-methylguanosine) cap is added to U2 cotranscriptionally, trimethylation being posttranscriptional. The immediate 5' flanking sequences have no influence on trimethylation; furthermore, trimethylation is not affected by changing the position and sequence of the cap site. The efficiency of trimethylation is reduced by deleting the Sm binding site from U2 RNA, but it is not altered by other mutations in the coding sequence. Insertion of artificial Sm binding sites either into a mutant U2 from which the natural binding site has been deleted or into SP6 transcripts generated in vitro allows these RNAs to become trimethylated. The trimethylase activity in Xenopus laevis oocytes is cytoplasmic.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
905-11
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
pubmed:publicationType
Journal Article