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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1986-8-15
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pubmed:abstractText |
We have investigated the role of poly(ADP)-ribosylation of adenoviral proteins in virus infection. Viral core proteins V and the precursor to protein VII were shown to be in vivo and in vitro acceptors of ADP-ribose. In vivo ADP-ribosylation was restricted to viral proteins as the histones were not labeled during the late phase of infection. The ADP-ribosylated core proteins were assembled into mature virus particles. In vitro ADP-ribosylation of adenoviral core proteins performed with purified poly(ADP-ribose) polymerase led to relaxation of the chromatin structure of both ts1 and wild type pyridine cores and pentonless particles and triggered the complete dissociation of wild type particles. A critical role for poly(ADP)-ribosylation in virus infection was confirmed by measuring the effect of the inhibitors 3-aminobenzamide and nicotinamide on virus particle yield and infectivity. Both inhibitors depressed particle yield by up to 9-fold, but infectivity was reduced by up to 10(4)-fold. These results suggest that ADP-ribosylation of adenovirus core proteins may have a role in virus decapsidation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0168-1702
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
313-29
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2941933-Adenosine Diphosphate Ribose,
pubmed-meshheading:2941933-Adenoviridae,
pubmed-meshheading:2941933-Benzamides,
pubmed-meshheading:2941933-Cell Line,
pubmed-meshheading:2941933-Cell Nucleus,
pubmed-meshheading:2941933-Chromatin,
pubmed-meshheading:2941933-DNA, Viral,
pubmed-meshheading:2941933-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2941933-Humans,
pubmed-meshheading:2941933-Microscopy, Electron,
pubmed-meshheading:2941933-Niacinamide,
pubmed-meshheading:2941933-Nucleoside Diphosphate Sugars,
pubmed-meshheading:2941933-Viral Core Proteins,
pubmed-meshheading:2941933-Virion
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pubmed:year |
1986
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pubmed:articleTitle |
Possible role of ADP-ribosylation of adenovirus core proteins in virus infection.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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