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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1986-5-12
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pubmed:abstractText |
Myotoxin a is a muscle-damaging toxin isolated from the venom of Crotalus viridis viridis. Its interaction with the Ca2+-ATPase of sarcoplasmic reticulum (SR) vesicles purified from rabbit skeletal muscle was investigated. Myotoxin a inhibited Ca2+ loading and stimulated Ca2+-dependent ATPase without affecting unidirectional Ca2+ efflux. Its action was dose, time, and temperature dependent. Myotoxin a partially blocked the binding of specific anti-(rabbit SR Ca2+-ATPase) antibodies. It is concluded that myotoxin a attaches to the SR Ca2+-ATPase and uncouples Ca2+ uptake from Ca2+-dependent ATP hydrolysis. Myotoxin a also prevented the formation of decavanadate-induced two-dimensional crystalline arrays of the SR Ca2+-ATPase.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0003-9861
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
246
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
90-7
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:2938545-Animals,
pubmed-meshheading:2938545-Calcium,
pubmed-meshheading:2938545-Calcium-Transporting ATPases,
pubmed-meshheading:2938545-Crotalid Venoms,
pubmed-meshheading:2938545-Crystallization,
pubmed-meshheading:2938545-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2938545-Microscopy, Electron,
pubmed-meshheading:2938545-Polymers,
pubmed-meshheading:2938545-Protein Binding,
pubmed-meshheading:2938545-Rabbits,
pubmed-meshheading:2938545-Sarcoplasmic Reticulum,
pubmed-meshheading:2938545-Temperature,
pubmed-meshheading:2938545-Vanadates,
pubmed-meshheading:2938545-Vanadium
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pubmed:year |
1986
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pubmed:articleTitle |
Interaction of myotoxin a with the Ca2+-ATPase of skeletal muscle sarcoplasmic reticulum.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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