Linked Life Data

293751

Source:http://linkedlifedata.com/resource/pubmed/id/293751

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1980-3-27
pubmed:abstractText
Egg-laying hormone (ELH), a neuropeptide synthesized by the bag cell neurons, induces egg laying and its correlated behavior in Aplysia californica. In the present study, ELH has been purified to homogeneity and its primary structure has been determined. We find this molecule to have 36 amino acid residues with a M(r) of 4385 and a calculated isoelectric point of 9.7. Direct microsequence analysis revealed a single amino acid sequence that is in agreement with the amino acid composition determined after acid hydrolysis of ELH: H-Ile-Ser-Ile-Asn-Gln-Asp-Leu-Lys-Ala-Ile-Thr-Asp-Met-Leu-Leu-Thr-Glu-Gln- Ile-Arg-Glu-Arg-Gln-Arg-Tyr-Leu-Ala-Asp-Leu-Arg-Gln-Arg-Leu-Leu-Glu-Lys-OH. Enzyme data indicate that the COOH-terminal lysine may be modified but its exact nature remains to be determined. There is no similarity between the amino acid sequence of ELH and that of presently known vertebrate neuropeptides. The two-step purification procedure, starting with a homogenate of bag cell clusters, consisted of cation exchange chromatography on SP C25 (Sephadex) followed by gel filtration on Bio-Gel P-6. Our purification results in a 100-fold enrichment of ELH from bag cell homogenates and a 36% recovery of purified radiolabeled marker ELH. Analysis of purified ELH radiolabeled with [(35)S]methionine or [(3)H]leucine on isoelectric focusing gels and on 8 M urea/sodium dodecyl sulfate gels showed only a single peak containing 90% of the radiolabel. Radiolabeled ELH migrated with a pI of 9.0-9.2 and an apparent M(r) of 3500-5700. ELH retained egg-laying bioactivity when eluted from this segment of the gel. We find that 2.5 nmol of pure ELH consistently induces egg laying at 20 degrees C.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/293751-17783250, http://linkedlifedata.com/resource/pubmed/commentcorrection/293751-4101989, http://linkedlifedata.com/resource/pubmed/commentcorrection/293751-4611885, http://linkedlifedata.com/resource/pubmed/commentcorrection/293751-5042022, http://linkedlifedata.com/resource/pubmed/commentcorrection/293751-5478254, http://linkedlifedata.com/resource/pubmed/commentcorrection/293751-5485408, http://linkedlifedata.com/resource/pubmed/commentcorrection/293751-5624338, http://linkedlifedata.com/resource/pubmed/commentcorrection/293751-571008, http://linkedlifedata.com/resource/pubmed/commentcorrection/293751-667015, http://linkedlifedata.com/resource/pubmed/commentcorrection/293751-877582, http://linkedlifedata.com/resource/pubmed/commentcorrection/293751-956770
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6656-60
pubmed:dateRevised
2010-9-2
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
Purification and primary structure of the neuropeptide egg-laying hormone of Aplysia californica.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.